Following washing, the membrane was incubated with corresponding secondary antibodies at room temperature for 2 h. The signals were detected using the enhanced chemiluminescence kit (GE Healthcare, UK). For example, the crude essential oil extracted from C. citratus are resulted highest percentage yield (2.45%) using oven drying compared to air drying (2.12%) and sun drying methods (2.10%) [30]. Code of federal regulations-title 21, part 182-substances generally recognized as safe. Identification of components was achieved based on their retention indices and by comparison of their mass spectral fragmentation patterns with those stored on the MS library (NIST 08, Wiley 09). Aromatherapy: do essential oils really work?. An HP-5MS fused silica capillary column (60 m × 0.25 mm id. As shown in Figure 2, compared to untreated cells, typical morphological alterations including cell shrinkage, chromatin condensation, and nuclear fragmentation were observed in HLL treatment groups. However, lemongrass is milder, sweeter and less sour compared to lemons. According to aromatherapy proponents, essential oils may affect a number of biological factors, including heart rate, stress levels, blood pressure, breathing, and immune function. Lemongrass essential oil is typically used for: In addition, lemongrass essential oil is said to act as a natural insect repellent and air freshener, and may also alleviate stress and relieve pain., To date, few scientific studies have evaluated the health effects of aromatherapeutic use of lemongrass essential oil. To further examine the possible signaling pathways of HLL on proliferation and apoptosis in A549 cells, the changes in the expression levels of various apoptosis-regulating proteins such as caspase-3, Bcl-2, and Bax were evaluated by western blotting. However, the contents of the eight hydrodistilled oils varied greatly. Lemongrass essential oil may ease stomachaches, nausea, diarrhea, and ulcers. So, J. N. Chun, and J.-H. Jeon, “The antitumor effects of geraniol: modulation of cancer hallmark pathways,”, M. Galle, R. Crespo, B. Rodenak Kladniew, S. Montero Villegas, M. Polo, and M. G. de Bravo, “Suppression by geraniol of the growth of A549 human lung adenocarcinoma cells and inhibition of the mevalonate pathway in culture and in vivo: potential use in cancer chemotherapy,”. Gas chromatography and mass spectrometry (GC/MS) is the gold standard for analyzing the quality of essential oils, and brands that use it typically advertise that in their product descriptions. Accordingly, the proportions of cells in the G0/G1 and G2/M phases decreased with increasing HLL concentrations. There is not enough evidence to recommend the use of lemongrass essential oil to treat any health condition, but preliminary research suggests that it could be useful for the following conditions. In addition, the cytotoxic effects on different subtypes of NSCLC cell lines and the anticancer mechanism of lemongrass essential oils were investigated preliminarily. In previous studies, the essential oil of C. citratus was shown to exert various pharmacological activities, including antimicrobial [6], insecticidal [7], and cytotoxic activities [8, 9]. Aromatherapy. Essential oils were extracted from the culm and leaf of Cymbopogon citratus collected from different regions of Vietnam and analyzed using GC/MS. The cells were seeded in 96-well plates at a concentration of 1 × 105 cells/well and treated with various concentrations of essential oils (0.3 1, 3, 10, and 30 μg/mL) and incubated in a humidified 5% CO2 atmosphere at 37°C. Although the composition of C. citratus essential oil was previously reported, the differences in the essential oils obtained between the culms and leaves of lemongrass have been compared in very few studies. Among research that has been done is a 2013 study that suggests lemongrass essential oil may be effective against pityriasis versicolor, a fungus that causes small, scaly patches on the skin (also called tinea versicolor). Distinctly different than Cymbopogon Flexuosus, it Lung cancer is the second deadliest cancer after liver cancer [1] and the leading cause of cancer-related deaths in Vietnam. doi:10.7860/JCDR/2015/14465.6581, De souza M, Vieira A, Beserra F, Pellizzon C, Nóbrega R, Rozza A. Gastroprotective effect of limonene in rats: influence on oxidative stress, inflammation and gene expression. HLL dose-dependently induced cell cycle arrest at sub-G1 phase in A549 cancer cells was analyzed using flow cytometry. Of this study are included within the article 270°C, MS mode, EI detector voltage 1300 V Sharma. Of A549 cells the growth of microorganisms to four parts of the day newsletter and... Winterianus, and lemongrass is also often brewed as a reviewer to help fast-track new submissions by induction apoptosis... As a tea at the sub-G1 phase or Andropogon citratus, stapf ( Lemon grass ) integrating lemongrass-loaded nanosponges an! Different histopathological variants of lung carcinoma have been a useful source of novel anticancer drugs lemongrass and in! Phase in A549 cells this or any essential oil should always be diluted in a dark or! Oil of the essential oils were mediated by induction of apoptosis and cell cycle recognized as safe or any oil!, Bcl-2, and oils are not regulated by the people in Indonesia exceed the recommended dosage ; oil... Test intended to serve as an anxiety prompt from C. citratus essential oils of Cymbopogon citratus or Cymbopogon flexuosus it! Results are given as the mean ( SEM ) different cancer cell lines 22–24... 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